Monoclonal ANTI-GST-Peroxidase (HRP) Conjugated 

QUANTITY: 100µl 

ISOTYPE: Mouse IgG2b 

DESCRIPTION: Monoclonal antibodies are produced by immunizing mouse with GST protein.

APPLICATION: Western Blot FORMAT: 1×PBS with 50% glycerol 

RECOMMENDED ANTIBODY DILUTION: Western Blot: 1:1000 

  1                     2 

Figure 1: Western Blot analysis of cell lystae (lane 1) and cell lysate expressing GST 

fusion protein (lane 2). 

STORAGE/HANDLING: Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycles. 

USAGE: FOR RESEARCH USE ONLY. NOT FOR HUMAN USE. 

Background: 

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B and eluting out the GST fusion protein from the column with denaturing form of glutathione. Using an anti-GST-peroxidase conjugated antibody provides a simple solution to detect the expression of GST fusion protein. 

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